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Background: A type of delivery represents an epigenetic factor with potential to affect further development by multiple mechanisms. One of them can be DNA methylation, which represents major epigenetic mechanism inolving direct chemical modification to the DNA. This study aims to examine wheather type of delivery induces changes of DNA methylation comparing the umbilical cord blood and periphery blood of the newborns. Methods: The blood samples from infants, born by vaginal delivery and cesarean section, were used to preparation of the Methylseq library according to NEBNext enzymatic Methyl-seq Methylation Library Preparation Kit with further generation of target-enriched DNA libraries by Twist Human Methylome Panel. DNA methylation status was measured using Illumina next-generation sequencing. Results: We identified 168 differentially methylated regions in umbilical cord blood samples and 157 regions in peripheral blood samples. These were associated with 59 common biological, metabolic and signaling pathways for umbilical cord and peripheral blood samples. Conclusions: Cesarean section probably represents important epigenetic factor with potential to induce changes in genome, which could play an important role in development of broad spectrum of disorders. Our results could contribute to elucidate how epigenetic factors, like type of delivery, could have an adverse impact on health later in the life. |
