Removal of fibroblasts from primary cultures of squamous cell carcinoma of the head and neck

Autor:	Michael L. Clawson, Theresa H. Piela-Smith, Bernard Greenberg, Lei L. Chen, Jeffrey D. Spiro, Craig D. Friedman, Eric A. Mann
Rok vydání:	1993
Předmět:	biology medicine.drug_class Cell Biology Monoclonal antibody Stain Molecular biology Pathology and Forensic Medicine Staining Cytokeratin medicine.anatomical_structure Cell culture Immunology medicine biology.protein Anatomy Antibody Fibroblast Head and neck
Zdroj:	Journal of Tissue Culture Methods. 15:1-9
ISSN:	1573-0603 0271-8057
DOI:	10.1007/bf02387282
Popis:	A commonly encountered problem in primary cultures of tumor specimens is overgrowth of fibroblastic cells. A method to remove fibroblasts from primary cultures of squamous cell carcinoma of the head and neck using immunomagnetic adsorption is described. A monoclonal antibody (BR2) was used for fibroblast depletion. BR2 stains human fibroblasts but does not stain human endothelial cells, human keratinocytes, or the squamous cell carcinoma lines, A-431 and NCI-H292. Eight primary cultures of head and neck tumor specimens were established. BR2-positive cells were removed from the suspension of cultured cells by immunomagnetic adsorption, which employs a magnetic iron oxide conjugated goat anti-mouse antibody as a secondary reagent. The BR2 positive cells became adherent to the surface of the culture dish which was placed over a magnet. BR2-negative cells remained in suspension and were collected for further culture or study. These BR2-negative cells were shown by cytokeratin staining to be epithelial cells.
Databáze:	OpenAIRE
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