sp. F92 immobilized on polyurethane foam shows ability to degrade various petroleum products
Autor: | Hai-Meng Tan, Eugene Quek, Yen-Peng Ting |
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Rok vydání: | 2006 |
Předmět: |
chemistry.chemical_classification
Environmental Engineering Light crude oil Chromatography Waste management biology Renewable Energy Sustainability and the Environment business.industry Microorganism Bioengineering General Medicine Biodegradation biology.organism_classification complex mixtures Diesel fuel Bioremediation Hydrocarbon Petroleum product chemistry lipids (amino acids peptides and proteins) business Waste Management and Disposal Rhodococcus |
Zdroj: | Bioresource Technology. 97:32-38 |
ISSN: | 0960-8524 |
DOI: | 10.1016/j.biortech.2005.02.031 |
Popis: | This work reports on the immobilization and performance of a hydrocarbon-degrading microorganism on polyurethane foam (PUF) in the bioremediation of petroleum hydrocarbons. The ability of four different microorganisms to immobilize on PUF and to degrade various petroleum products (Arabian light crude (ALC), Al-Shaheen crude (ASC), diesel and oil slops) was assessed by measuring the n-alkane fraction remaining in the petroleum products over time. A Rhodococcus sp. (designated as F92) had the highest number of immobilized viable cells (10 9 cells per cm 3 PUF) and a maximum attachment efficiency of 90% on PUF of a density of 14 kg/m 3 . Scanning electron microscopy showed the presence of extracellular structures that could play an important role in the immobilization of F92 on PUF. Analysis by GC–MS revealed that both free and immobilized F92 cells were able to degrade approximately 90% of the total n-alkanes in the petroleum products tested within 1 week at 30 � C. Rhodococcus sp. F92 was efficiently immobilized onto PUF and the immobilized cells were able to degrade a variety of petroleum products such as ALC, ASC, diesel and oil slops. The results suggest the potential of using PUF-immobilized Rhodococcus sp. F92 to bioremediate petroleum hydrocarbons in an open marine environment. � 2005 Elsevier Ltd. All rights reserved. |
Databáze: | OpenAIRE |
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