Regulated Expression of Osteopontin in the Peri-Implantation Rabbit Uterus1
| Autor: | Maria J. Illera, Gary E. Olson, Martha H. Corjay, Kevin G. Osteen, Kim A. Boggess, Stan A. Beyler, Bruce A. Lessey, K.B.C. Apparao |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
biology medicine.drug_class Uterus Trophoblast Cell Biology General Medicine In situ hybridization Endometrium medicine.anatomical_structure Endocrinology stomatognathic system Reproductive Medicine Estrogen Internal medicine medicine biology.protein Blastocyst Osteopontin Northern blot |
| Zdroj: | Biology of Reproduction. 68:1484-1490 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod.101.001347 |
| Popis: | Blastocyst attachment to the lining of the mammalian uterus during early implantation involves the initial apposition of the trophoblast to the uterine epithelial surface. Osteopontin (OPN) is a glycoprotein component of the extracellular matrix that is secreted by the glandular epithelium of mammalian uteri at the time of implantation. This protein is recognized by several members of the integrin family and promotes cell-cell attachment and adhesion. In the present study, rabbit uteri were examined using Northern and in situ hybridization to evaluate the temporal and spatial distribution of OPN mRNA during early pregnancy. Northern blot analysis demonstrated a dramatic increase in OPN expression on Days 4‐7 of pregnancy, corresponding to the rise in circulating progesterone and the time of initial embryo attachment in this species. In situ hybridization analysis revealed OPN mRNA expression on Day 6.75 of pregnancy, which was most prominent on endometrial epithelium. Using immunofluorescence, OPN protein was present on the glandular epithelium on Day 6.75 of pregnancy, but was absent on blastocysts. Further, no expression of OPN mRNA or protein was found in the nonpregnant endometrium. Induction of endometrial OPN expression was observed in unmated rabbits treated with progesterone alone and was prevented by cotreatment with the antiprogestin ZK137.316. Estradiol-17b had no effect on OPN expression by itself, and estrogen priming was not necessary to demonstrate the stimulatory effect of progesterone. In The rabbit uterus, as in other mammalian species studied, OPN is expressed in a stage-specific manner by the endometrial 1Part of this work was presented at the 47th Annual Meeting of the Society for Gynecologic Investigation in Chicago, Illinois, March 22‐25, 2000. This research was supported by NICHD/NIH through cooperative agreement U54 HD-35041 (B.A.L.) and HD-37321 (K.G.O., G.E.O.) as part of the Specialized Cooperative Centers Program in Reproduction Research, the National Cooperative Program on Markers of Uterine Receptivity for Blastocyst Implantation (HD-34824; B.A.L.), and the Fogerty International Fellowship award (K.B.C.A.). |
| Databáze: | OpenAIRE |
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