| Autor: |
D. G. Steyn, H.H. Meissner, F.M. Hagg, C.H. Horn, P. H. Henning |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
Animal Feed Science and Technology. 157:13-19 |
| ISSN: |
0377-8401 |
| DOI: |
10.1016/j.anifeedsci.2009.12.011 |
| Popis: |
The objectives were: (a) to select the most effective lactate-utilizing Megasphaera elsdenii (Me) isolates (strains) from mixed rumen cultures (Phase 1) and (b) to determine in vivo the efficacy of the two most promising isolates from Phase 1 to control ruminal acidosis (Phase 2). In Phase 1 batch cultures of nine strains of lactate-utilizing Me were isolated and compared with the Me type strain ATCC 25940 and a membrane-filtered supernatant of a centrifuged culture (Control). Culture concentration in the incubation tubes was ca 5 × 105 cfu's/ml. In Phase 2 forage-fed rumen-fistulated sheep were drenched intra-ruminally 100 ml containing 1011 cfu's of the promising Me isolates CH4 and CH7, following abrupt administration of 1 kg maize meal and 300 g maltose per sheep, and compared with Control sheep drenched with a placebo. For Me isolates CH3, CH4 and CH7 rumen pH remained below pH = 5.0 for 5 h/24 h for the type strain and the Control (p 5.0 (p 5.5 for the total post-drenching period. Lactic acid concentration post-drenching consistently remained 55 mmol/l at +10 h for Control (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full Text from ScienceDirect