Mouse lymphoma thymidine kinase gene mutation assay: Follow-up meeting of the international workshop on Genotoxicity testing—Aberdeen, Scotland, 2003—Assay acceptance criteria, positive controls, and data evaluation

Autor: Melvin Lloyd, Jenness B. Majeska, Robert R. Delongchamp, Takashi Omori, Leon F. Stankowski, Sai-Mei Hou, Colin Riach, B. Bhaskar Gollapudi, Peter Jenkinson, Julie Clements, Masamitsu Honma, Shinobu Wakuri, George Bolcsfoldi, Jane Clarke, Freddy Van Goethem, Maria A. Cifone, Brian Burlinson, Isao Yoshimura, Richard H.C. San, Michael D. Fellows, Robert Durward, Michael R. O’Donovan, Martha M. Moore, Ajit K. Thakur, Brian Myhr
Rok vydání: 2006
Předmět:
Zdroj: Environmental and Molecular Mutagenesis. 47:1-5
ISSN: 1098-2280
0893-6692
DOI: 10.1002/em.20159
Popis: The Mouse Lymphoma Assay (MLA) Workgroup of the International Workshop on Genotoxicity Testing (IWGT), comprised of experts from Japan, Europe, and the United States, met on August 29, 2003, in Aberdeen, Scotland, United Kingdom. This meeting of the MLA Workgroup was devoted to reaching a consensus on the appropriate approach to data evaluation and on acceptance criteria for both the positive and negative/vehicle controls. The Workgroup reached consensus on the acceptance criteria for both the agar and microwell versions of the MLA. Recommendations include acceptable ranges for mutant frequency, cloning efficiency, and suspension growth of the negative/vehicle controls and on criteria to define an acceptable positive control response. The recommendation for the determination of a positive/negative test chemical response includes both the requirement that the response exceeds a defined value [the global evaluation factor (GEF)] and that there also be a positive dose-response (evaluated by an appropriate statistical method).
Databáze: OpenAIRE
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