Current address: Shinji Tomita, MD, Cardiothoracic Surgical Unit, Level 4, Auckland City Hospital, Auckland, New Zealand
| Autor: | Chikao Yutani, Michiko Ishida, Yoshinori Ohtsu, Shinji Tomita, Shinya Fukuhara, Toshiya Fujisato, Soichiro Kitamura, Takeshi Nakatani |
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| Rok vydání: | 2005 |
| Předmět: |
musculoskeletal diseases
Genetically modified mouse Cardiac function curve medicine.medical_specialty Chemistry Angiogenesis musculoskeletal neural and ocular physiology Growth factor medicine.medical_treatment General Medicine musculoskeletal system Molecular biology humanities Green fluorescent protein medicine.anatomical_structure Internal medicine Drug delivery medicine Cardiology Bone marrow Cardiology and Cardiovascular Medicine Artery |
| Zdroj: | Circulation Journal. 69:850-857 |
| ISSN: | 1347-4820 1346-9843 |
| Popis: | BACKGROUND The present study examined whether a bioengineered polyglycolic acid cloth (PGAC) impregnated with bone marrow cells (BMC) improved the function and angiogenesis of the infarcted heart. METHODS AND RESULTS The coronary artery was ligated in Lewis rats and the infarcted area was covered with a PGAC in group 1 (n=8), with a PGAC containing basic-fibroblast growth factor (b-FGF) in group 2 (n=11) and a PGAC containing b-FGF and freshly isolated BMC in group 3 (n=10). In addition, BMC derived from transgenic mice expressing green fluorescent protein (GFP)-BMC were seeded into a PGAC, which was sutured over the infarcted area of C57BL/6 mice (n=5). In the rat study, developed and systolic pressures, dp/dt max and dp/dt min) were the highest in group 3, as were the capillary density in the PGAC and infarcted area. In the mouse study, there were few GFP-BMC in the PGAC, but none in the infarcted area. CONCLUSIONS A PGAC with BMC improved cardiac function by inducing angiogenesis without migration of BMC. Freshly isolated BMC work as angiogenic inducers and a PGAC is useful as a "drug delivery system". |
| Databáze: | OpenAIRE |
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