THU0054 Long non-coding rna gaplinc promotes proliferation and invasion of fibroblast-like synoviocytes as microrna sponging in ra patients
| Autor: | Xuan Bi, Y. Pan, Xi Qing Luo, Bi Yao Mo |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
030203 arthritis & rheumatology
0301 basic medicine medicine.diagnostic_test business.industry Cell RNA Transfection Long non-coding RNA Flow cytometry 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure microRNA Cancer research Medicine Viability assay KEGG business |
| Zdroj: | THURSDAY, 14 JUNE 2018. |
| DOI: | 10.1136/annrheumdis-2018-eular.3584 |
| Popis: | Background Accumulating evidence suggested that long non-coding RNAs (lncRNAs) play diverse functional roles in many autoimmune diseases including rheumatoid arthritis (RA). However, there is a dearth of knowledge in what role these transcripts play in fibroblast-like synoviocytes (FLSs) of RA patients. LncRNA GAPLINC, a novel long non-coding RNA, was first described in gastrointestinal cancer tissues and associated with bad behaviours of tumour cell as well as poor prognosis in patients. Objectives This study was undertaken to explore the expression and roles of LncRNA GAPLINC in RA-FLSs and investigate its possible mechanism. Methods RA-FLSs and trauma-FLSs were cultured from synovial specimens. The expression of RNA was detected by qRT-PCR. GAPLINC suppression was transfected by siRNA. Cell viability analysis was taken by CCK-8 assay and flow cytometry. Cell invasion was using transwell chamber methodology. The bioinformatics analysis was performed using miRanda, PITA, RNAhybrid algorithms, as well as KEGG and Gene Ontology(GO) analysis. Results The relative expression of LncRNA GAPLINC was significantly higher in RA-FLSs than trauma-FLSs (p Conclusions The results suggest that elevated LncRNA GAPLINC expression promote the proliferation and invasion of RA-FLSs and it may function as a novel microRNAs sponging agent. Additionally, LncRNA GAPLINC may regulate RA-FLS pathological behaviours in an miR-382–5 p-dependent and miR-575-dependent manner. Based upon these findings, lncRNA GAPLINC may provide a novel valuable therapeutic target for RA patients. Acknowledgements This work was supported by grants from Province Natural Science Fund of Guangdong, ChinaNo.2014A030313080) and National Natural Science Foundation of China (No.81771750). Disclosure of Interest None declared |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|