Influence of PDI Gene Overexpression on the Production of Heterologous Proteins in Yeast Pichia pastoris
| Autor: | M. A. Tsygankov, M. V. Padkina |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
biology Heterologous biology.organism_classification Yeast Pichia pastoris law.invention 03 medical and health sciences 030104 developmental biology Secretory protein Biochemistry law Genetics Recombinant DNA Animal Science and Zoology Secretion Protein disulfide-isomerase Agronomy and Crop Science Gene |
| Zdroj: | Russian Journal of Genetics: Applied Research. 8:197-205 |
| ISSN: | 2079-0600 2079-0597 |
| DOI: | 10.1134/s2079059718020120 |
| Popis: | The Pichia pastoris yeast is used for recombinant secretory proteins production. The overexpression of assistant genes, the coding proteins involved in secretion, is one of the approaches used to improve the production of target proteins. PpPDI gene encodes P. pastoris yeast protein disulfide isomerase (Pdi). The aim of our study is to evaluate the effect of Pdi overproduction on the production of recombinant interferons. We obtain the yeast Pichia pastoris strains expressing PpPDI gene under the control of alcohol oxidase-1 (AOX1) promoter. The effect of the overexpression of the PpPDI gene on the production of heterogious proteins—interferon-alfa16 and chicken interferon-gamma—and on the cell growth rate is shown. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink