Determination of Total Fumonisins in Corn by Competitive Direct Enzyme-Linked Immunosorbent Assay: Collaborative Study
| Autor: | Malone B, P. F. Ross, Mohamed Abouzied, Charles B. Bird, Eppley R, L. G. Rice |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Pharmacology
chemistry.chemical_classification Fusarium biology business.industry food and beverages Elisa assay Repeatability biology.organism_classification Analytical Chemistry Biotechnology chemistry.chemical_compound Enzyme chemistry Fumonisin Environmental Chemistry Food science Mycotoxin business Agronomy and Crop Science Analysis method Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 85:404-410 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.1093/jaoac/85.2.404 |
| Popis: | Fumonisins—mycotoxins produced by some Fusarium species—have been shown to be the causative agent of diseases in horses and other domesticated animals as well as possible carcinogens in humans. A collaborative study was conducted to evaluate the effectiveness of a competitive direct enzyme-linked immunosorbent assay (CD-ELISA) for the determination of total fumonisins (B1, B2, and B3) in corn. The test portion was extracted with methanol–water (7 + 3), filtered, diluted, and tested on the CD-ELISA. Naturally and artificially contaminated corn test portions were sent to 13 collaborators in the United States. Naturally contaminated field test portions were prepared at 3 different levels. Artificially contaminated test portions were spiked at 1.0, 3.0, and 5.0 mg/kg total fumonisins (B1, B2, and B3). Average recoveries of total fumonisins were 120, 100, and 90%, respectively. The relative standard deviations for repeatability ranged from 13.3 to 23.3% and the relative standard deviations for reproducibility ranged from 15.8 to 30.3% across all levels tested. HORRAT values, calculated for each individual sample, ranged from 1.24 to 1.94. This method demonstrated acceptable intra- and interlaboratory precision at the levels tested. |
| Databáze: | OpenAIRE |
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