Purification and characterization of xylanase isoenzymes from red palm weevil Rhynchophorus ferrugineus
| Autor: | Manal M.E. Ghanem, Ahmed M. Abd-Elaziz, Magda A. Mohamed, Ibrahim M. Shams-Eldin |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine chemistry.chemical_classification biology Chemistry Active site Bioengineering biology.organism_classification 01 natural sciences Applied Microbiology and Biotechnology Xylan Enzyme assay 010602 entomology 03 medical and health sciences Rhynchophorus Hydrolysis 030104 developmental biology Enzyme Xylanase biology.protein Specific activity Food science Agronomy and Crop Science Food Science Biotechnology |
| Zdroj: | Biocatalysis and Agricultural Biotechnology. 14:321-327 |
| ISSN: | 1878-8181 |
| Popis: | Xylanase activity in the guts of Rhynchophorus ferrugineus during larval development had been investigated. The activity in the guts from the 5th to the 12th instars ranged from 12.5 to 67 U/gut with specific activity ranged from 50 to 124 U/mg protein. The 10th instars larvae had the highest enzyme activity. Purification of two predominant xylanase isoenzymes was performed by gel filtration on Sephacryl S-200 and chromatography on DEAE-Sepharose. XyI and XyII had specific activities of 468.6 and 402 U/mg proteins, native molecular weights of 25 and 42 kDa, respectively. They had monomeric forms, showed identical optimum activity at pH 5.5 and 40 °C. XyII exhibited higher thermal stability and activation energy than XyI. Inhibition by Hg2+, Cu2+, EDTA and stimulation by dithiol-reducing agents revealed the presence of at least one sulfhydryl group in the active site of the enzyme and they were metalloenzyme. They exhibited high specificity towards natural xylans. They produced xylotriose, xylotetraose and xylopentaose as the main hydrolysis products of beechwood xylan. The results were compared with those previously recorded for different insect species. |
| Databáze: | OpenAIRE |
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