Blood Neutrophils In COPD But Not Asthma Exhibit A Primed Phenotype With Downregulated CD62L Expression
| Autor: | Michael Fricker, Katherine J. Baines, Ravi Lokwani, Jodie L. Simpson, Daniel Barker, Peter A. B. Wark |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
COPD
biology Cell adhesion molecule business.industry CD11c chemical and pharmacologic phenomena hemic and immune systems General Medicine CD16 Systemic inflammation medicine.disease Respiratory burst 03 medical and health sciences 0302 clinical medicine 030228 respiratory system Integrin alpha M Immunology medicine biology.protein 030212 general & internal medicine medicine.symptom business Asthma |
| Zdroj: | International Journal of Chronic Obstructive Pulmonary Disease. 14:2517-2525 |
| ISSN: | 1178-2005 |
| Popis: | Purpose To characterize neutrophils in obstructive airway disease by measuring their surface adhesion molecules and oxidative burst along with characterizing them into different subsets as per their adhesion molecule expression. Patients and methods Peripheral blood from adults with COPD (n=17), asthma (n=20), and healthy participants (n=19) was examined for expression of CD16, CD62L, CD11b, CD11c, and CD54, and analyzed by flow cytometry. For oxidative burst and CD62L shedding analysis, CD16 and CD62L stained leukocytes were loaded with Dihydrorhodamine-123 (DHR-123) and stimulated with N-Formylmethionine-leucyl-phenylalanine (fMLF). Neutrophil subsets were characterized based on CD16 and CD62L expression. Marker surface expression was recorded on CD16+ neutrophils as median fluorescence intensity (MFI). Results Neutrophil surface expression of CD62L was significantly reduced in COPD (median (IQR) MFI: 1156 (904, 1365)) compared with asthma (1865 (1157, 2408)) and healthy controls (2079 (1054, 2960)); p=0.028. COPD neutrophils also demonstrated a significant reduction in CD62L expression with and without fMLF stimulation. Asthma participants had a significantly increased proportion and number of CD62Lbright/CD16dim neutrophils (median: 5.4% and 0.14 × 109/L, respectively), in comparison with healthy (3.54% and 0.12 × 109/L, respectively); p Conclusion Reduced CD62L expression suggests blood neutrophils have undergone priming in COPD but not in asthma, which may be the result of systemic inflammation. The increased shedding of CD62L receptor by COPD blood neutrophils suggests a high sensitivity for activation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|