P1‐029: A comparison of conventional versus push‐pull microdialysis for the detection of amyloid β in the mouse brain
| Autor: | Thomas I. F. H. Cremers, Robert Brendza, Ruth Motter, Robert Freije, Marieke G. C. van der Hart, Isaac Veinbergs, Lisa Yu, Harm Kooijker, Pearl Tanaka, Gunnar Flik |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Microdialysis
Amyloid β Physiological significance Epidemiology Chemistry Health Policy Substrate (chemistry) Cleavage (embryo) Psychiatry and Mental health Cellular and Molecular Neuroscience Developmental Neuroscience Biophysics Potency Neurology (clinical) Geriatrics and Gerontology Push pull Gamma secretase |
| Zdroj: | Alzheimer's & Dementia. 7 |
| ISSN: | 1552-5279 1552-5260 |
| DOI: | 10.1016/j.jalz.2011.05.309 |
| Popis: | using a sensitive plate based ELISA system (MSD). Results: Unlike gamma secretase inhibitors, which demonstrate a “potency shift" when the concentration of substrate is very high as in systems overexpressing APP (Burton et al 2008), the potency of GSMs does not demonstrate a systematic shift in overexpressing systems. The pattern of modulation of As peptides was consistent across model systems and specific to each structurally distinct GSM, with some demonstrating a decrease in As42 and an increase in As38 and others demonstrating a decrease in both As42 and 38. Conclusions: The physiological significance of these alterations in cleavage site is not well understood, but appears to be driven by unique interactions of GSMs with gamma secretase. |
| Databáze: | OpenAIRE |
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