The dds20 + Gene Controls a Novel Rad51Sp-Dependent Pathway of Recombinational Repair in Schizosaccharomyces pombe

Autor: F. K. Khasanov, Vladimir I. Bashkirov, A. F. Salakhova, Chepurnaia Ov, Galina V. Savchenko, Korolev Vg
Rok vydání: 2005
Předmět:
Zdroj: Russian Journal of Genetics. 41:593-601
ISSN: 1608-3369
1022-7954
DOI: 10.1007/s11177-005-0132-7
Popis: Repair of DNA double-strand break (DSB) is an evolutionary conserved Rad51-mediated mechanism. In yeasts, Rad51 paralogs, Saccharomyces cerevisiae Rad55-Rad57 and Schizosaccharomyces pombe Rhp55-Rhp57 are mediators of the nucleoprotein Rad51 filament formation. As shown in this work, a novel Rad51Sp-dependent pathway of DSB repair acts in S. pombe parallel to the pathway mediated by Rad51 paralogs. A new gene dds20+ that controls this pathway was identified. The overexpression of dds20+ partially suppresses defects of mutant rhp55Δ in DNA repair. Cells of dds20Δ manifest hypersensitivity to a variety of genotoxins. Epistatic analysis revealed that dds20+ is a gene of the recombinational repair group. The role of Dds20 in repair of spontaneous damages occurring in the process of replication and mating-type switching remains unclear. The results obtained suggest that Dds20 has functions beyond the mitotic S phase. The Dds20 protein physically interacts with Rhp51(Rad51Sp). Dds20 is assumed to operate at early recombinational stages and to play a specific role in the Rad51 protein filament assembly differing from that of Rad51 paralogs.
Databáze: OpenAIRE
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