Metabolomics and lipidomics analyses delineating Hfq deletion- induced metabolic alterations in Vibrio alginolyticus
| Autor: | Mudan Xu, Heyang Jiang, Jieyu Zhao, Jiamin Ren, Huan Liu, Yanni Zhao, Xuefeng Chen, Hua Li, Li Yue, Chen Dan, Zhang Kun |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Vibrio alginolyticus 0303 health sciences biology Catabolism Lysophosphatidylethanolamine 04 agricultural and veterinary sciences Phosphatidic acid Aquatic Science biology.organism_classification Amino acid 03 medical and health sciences chemistry.chemical_compound chemistry Biochemistry Lipidomics 040102 fisheries Aromatic amino acids Cardiolipin 0401 agriculture forestry and fisheries 030304 developmental biology |
| Zdroj: | Aquaculture. 535:736349 |
| ISSN: | 0044-8486 |
| DOI: | 10.1016/j.aquaculture.2021.736349 |
| Popis: | Vibrio alginolyticus, a Gram-negative halophilic zoonotic pathogen, can cause bacterial hemorrhagic septicemia in various aquaculture species and wound infections in humans. V. alginolyticus brings about huge economic losses in aquaculture all around the world. Hfq, a ubiquitous RNA-binding protein, is a global post-transcriptional regulator in most bacteria and some archaea, by facilitating base-pairing between sRNAs and their mRNA targets, and is essential for adaptation to different environments and growth conditions. Despite its important roles in the stress adaptation, virulence, nutrient utilization, and colony morphology in V. alginolyticus, further research is required to shed light on the pathways modulated by Hfq. In this study, we used metabolomics and lipidomics analytical approaches based on ultrahigh-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS) to understand the impact of Hfq on the metabolic profile of V. alginolyticus. The deletion of the hfq gene resulted in the growth deficiency and cell elongation of V. alginolyticus cultured in the rich medium. Substantial metabolic alterations, mainly involved in amino acids, nucleotides, fatty acids, and lipids, were observed in the hfq deletion mutant strain (Δhfq) when compared to the wild-type (WT) strain and its complemented strain (hfq+). Notably, the hfq deletion induced accumulations of free fatty acids (FFAs) and nucleotides, but decreased levels of most amino acids (e.g., branched amino acids, aromatic amino acids, and aspartate family amino acids). Meanwhile, the majority of lipids (e.g., lysophosphatidylglycerol, lysophosphatidylethanolamine, phosphatidylcholine, phosphatidic acid, cardiolipin, sphingomyelin, some phosphatidylglycerol and phosphatidylethanolamine with mono- or poly-unsaturated fatty acids) were decreased, while ceramide, diacylglycerol, triacylglycerol and some phosphatidylglycerol and phosphatidylethanolamine with saturated fatty acids increased in the Δhfq strain. These data demonstrated that the hfq deletion triggered de novo synthesis of FFAs and nucleosides and enhanced the catabolism of phospholipid and sphingomyelin to further regulate cell division and growth. Additionally, the hfq deletion could inhibit the synthesis of the branched amino acids, aromatic amino acids and enhance the TCA cycle to influence cell stress defenses and energy metabolism. Our research indicates that Hfq can regulate pathways associated with cell division, growth and stress resistance, being an important metabolic modulator in V. alginolyticus. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect