| Autor: | Bernard F. Adamitsch, Werner A. Hampel |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
chemistry.chemical_classification
Growth medium biology Triacylglycerol lipase Bioengineering Brevibacterium General Medicine biology.organism_classification Applied Microbiology and Biotechnology chemistry.chemical_compound Enzyme chemistry Biochemistry biology.protein Specific activity Lipase Lysozyme Bacteria Biotechnology |
| Zdroj: | Biotechnology Letters. 22:1643-1646 |
| ISSN: | 0141-5492 |
| DOI: | 10.1023/a:1005633828125 |
| Popis: | When Brevibacterium linens ATCC 9172 was grown in shake flasks, it produced a cell-associated lipase with a specific activity of 152 to 188 U g−1 cells depending on the composition of the growth medium. There was no growth in media containing tributyrine as the sole carbon source. The cell-associated lipase had maximum activity at pH 8.0 and 37 °C and was strongly inhibited by 3,4-dichloroisocoumarin, an inhibitor specific for serine esterases. Cell-associated activity was released from the cells by treatment with lysozyme. The kinetics of lipase formation was closely related to the amount of biomass formed during growth. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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