EXPRESSION OF RENAL COLLECTING DUCT AMILORIDE-SENSITIVE Na CHANNEL SUBUNIT GENES DURING MATURATION. † 2136

Autor:	Mary C Carmichael, Jennifer Manning, V. Matti Vehaskari
Rok vydání:	1996
Předmět:	Epithelial sodium channel Messenger RNA biology Protein subunit Molecular biology Reverse transcriptase Amiloride Pediatrics Perinatology and Child Health Agarose gel electrophoresis medicine biology.protein Gene Glyceraldehyde 3-phosphate dehydrogenase medicine.drug
Zdroj:	Pediatric Research. 39:359-359
ISSN:	1530-0447 0031-3998
DOI:	10.1203/00006450-199604001-02160
Popis:	The rate-limiting step in Na transport by the renal cortical collecting duct is the apical amiloride-sensitive Na channel (ENaC), which is composed of three recently identified subunits (α, β, and γ). The immature CCD has a low rate of active Na transport and a very low Na-channel activity. To explore the mechanism, expression of the three ENaC subunit genes was studied in rat kidney at different developmental stages. Reverse transcription followed by polymerase chain reaction (rt-pcr) and agarose gel electrophoresis was used for specific mRNA detection. GAPDH mRNA, which did not show any age-related changes, was used as a positive control. All products were also partially sequenced to confirm their identity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::91cdb3c5f498929eec1e2d3cd2742009 https://doi.org/10.1203/00006450-199604001-02160 Zobrazit plný text záznamu