Genetic Diversity of the Endemic Xantus’ Hummingbird Using 16 Novel Polymorphic Microsatellite Loci, and Their Cross Amplification between Six Related Species
| Autor: | Oscar Adrián Lozano-Garza, Cristina González-Rubio Sanvicente, Francisco J. García-De-León, Ricardo Rodríguez-Estrella |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Genetics Linkage disequilibrium education.field_of_study Genetic diversity biology Hylocharis xantusii Population Population genetics biology.organism_classification 03 medical and health sciences 030104 developmental biology Calypte costae biology.animal Microsatellite Hummingbird education |
| Zdroj: | Open Journal of Genetics. :19-27 |
| ISSN: | 2162-4461 2162-4453 |
| DOI: | 10.4236/ojgen.2016.61003 |
| Popis: | A set of 16 microsatellite loci was developed and characterized for the Xantus’ hummingbird (Hylocharis xantusii) by using 454 next-generation sequencing. Twenty-five H. xantusii samples from one population were genotyped; all loci were polymorphic, with the number of alleles ranging from three to ten. The mean observed heterozygosity was 0.681 for all loci. No significant linkage disequilibrium was detected, but five loci (Hxan05, 06, 09, 13 and 14) showed deviation from Hardy-Weinberg equilibrium. These microsatellite loci are the first to be characterized for H. xantusii. A moderate to high level of cross-species amplification was observed across the six hummingbird species (31% - 87.5%), with the best cross amplification results observed in the closest related species (H. leucotis, Cynanthus latirostris, Calypte costae). The availability of these molecular tools allows assessing questions integrating population genetics, ecology, conservation, and evolutionary history for H. xantusii and for other phyogenetically related species. |
| Databáze: | OpenAIRE |
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