A highly sensitive and stable detection of acetylcholine by HPLC-osmium-horseradish peroxidase redox polymer electrode coated on a gold radial flow ring disk
| Autor: | Takeshi Kato, Katsunobu Yamamoto, Kentaro Sato, Toshiyuki Chikuma |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Chromatography
biology Chemistry chemistry.chemical_element Biochemistry Redox Acetylcholinesterase High-performance liquid chromatography Horseradish peroxidase Analytical Chemistry chemistry.chemical_compound Electrode medicine biology.protein Environmental Chemistry Cholinergic Osmium Spectroscopy Acetylcholine medicine.drug |
| Zdroj: | Analytica Chimica Acta. 521:209-213 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2004.06.062 |
| Popis: | To detect a low level of acetylcholine (Ach) output in the dialysate of rat frontal cortex, osmium-peroxidase redox polymer modified gold-ring disk electrode (Os-HRP-RDE) was fabricated in high-performance liquid chromatography (HPLC)-electrochemical detection (ECD). In comparison with platinum electrode, the sensitivity of acetylcholine detection by Os-HRP-RDE was stable during a day, the coefficient of variation was 0.95%, and the decrease of the sensitivity for 1 week was 5.4%. The Os-HRP-RDE could detect lower than 3 fmol of acetylcholine. The present technique, in the absence of acetylcholinesterase (AchE) inhibitor, is useful to facilitate physiological investigations of cholinergic neuronal activity in the brain. |
| Databáze: | OpenAIRE |
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