Discovery and Characterization of a Novel Method for Effective Improvement of Cyclodextrin Yield and Product Specificity
| Autor: | Hua Jinghan, Jing-wen Yang, Hong-bin Zhang, Hao Wu, Jiadong Wang, Xue-qin Hu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
biology Cyclodextrin Starch Bacillus cereus Alcohol 02 engineering and technology General Chemistry Enzymatic synthesis 010402 general chemistry 021001 nanoscience & nanotechnology biology.organism_classification 01 natural sciences Combinatorial chemistry 0104 chemical sciences chemistry.chemical_compound chemistry Drug delivery 0210 nano-technology Selectivity Tertiary alcohols |
| Zdroj: | Chemical Research in Chinese Universities. 35:708-713 |
| ISSN: | 2210-3171 1005-9040 |
| DOI: | 10.1007/s40242-019-8406-0 |
| Popis: | Cyclodextrins(CDs) are widely used in food, pharmaceuticals, drug delivery, and chemical industries and in agriculture and environmental engineering. To improve the yield and selectivity of CDs, this work presented a facile, scalable and efficient enzymatic synthesis of β-CD from starch using β-cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) from Bacillus cereus. First, we found that the pretreatment of starch dramatically influenced CDs yield that was related to the structure and molecular weight of the substrate starch. Second, alcohol solvents influenced the yield and product selectivity of CDs; tertiary alcohols enhanced CDs yield(from 54.95% to 68.21%) and secondary alcohols increased the product selectivity(β-CD/γ-CD changed from 6.25 to 8.05). Fluorescence quenching analysis showed that the binding constants and entropy of the solvents influenced the yield and product selectivity, respectively. In conclusion, the results demonstrate that this study provides a promising method for the industrial production of β-CD. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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