Protective Effect of Radiation-induced New Blackberry Mutant γ-B201 on H2O2-induced Oxidative Damage in HepG2 Cells
| Autor: | Il-Yun Jeong, Chang-Hyun Jin, Yangkang So, Jong Chun Park, Yong-Wook Jeong, Byoung Ok Cho, Chang Wook Lee, Hong-Sun Yook, Myung-Woo Byun |
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| Rok vydání: | 2014 |
| Předmět: |
chemistry.chemical_classification
Reactive oxygen species DNA damage Biology medicine.disease medicine.disease_cause Applied Microbiology and Biotechnology Molecular biology Superoxide dismutase Comet assay chemistry Biochemistry Catalase biology.protein medicine Viability assay Cell damage Oxidative stress Food Science Biotechnology |
| Zdroj: | Korean Journal of Food Science and Technology. 46:384-389 |
| ISSN: | 0367-6293 |
| DOI: | 10.9721/kjfst.2014.46.3.384 |
| Popis: | The objective of the present study was to investigate the chemical composition of anthocyanin-enriched extract of radiation-induced blackberry (Rubus fruticosus L.) mutant (γ-B201) as well as the protective effect of γ-B201 against oxidative stress in vitro. The cytotoxicity, reactive oxygen species (ROS) scavenging capacity, and DNA damage were assessed by WST-1 assay, flow cytometry, and comet assay, respectively. Lactate dehydrogenase, superoxide dismutase, and catalase activities were determined by using a commercial kit. The in vitro results showed that γ-B201 increased the cell viability, reduction of lactate dehydrogenase release, and intracellular ROS scavenging capacity in hydrogen peroxide (H 2 O 2 )-treated HepG2 cells. Furthermore, treatment with γ-B201 attenuated DNA damage in H 2 O 2 -treated HepG2 cells and treatment with γ-B201 restored the activity of superoxide dismutase and catalase in H2O2-treated HepG2 cells. In conclusion, the present study suggests that γ-B201 blackberry extract can exert a significant cytoprotective effect against H2O2-induced cell damage. |
| Databáze: | OpenAIRE |
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