An Integrated Experimental and Computational Approach for Characterizing the Kinetics and Mechanism of Triadimefon Racemization
| Autor: | Quincy Teng, Caleb M. Dillingham, Satori A. Marchitti, Qianyi Cheng, John F. Kenneke |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Pharmacology
education.field_of_study Stereochemistry Organic Chemistry Population 010501 environmental sciences 010402 general chemistry 01 natural sciences Catalysis 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound Triadimefon chemistry Deuterium Computational chemistry Drug Discovery Kinetic isotope effect Racemic mixture Enantiomer Chirality (chemistry) education Racemization Spectroscopy 0105 earth and related environmental sciences |
| Zdroj: | Chirality. 28:633-641 |
| ISSN: | 0899-0042 |
| Popis: | Enantiomers of chiral molecules commonly exhibit differing pharmacokinetics and toxicities, which can introduce significant uncertainty when evaluating biological and environmental fates and potential risks to humans and the environment. However, racemization (the irreversible transformation of one enantiomer into the racemic mixture) and enantiomerization (the reversible conversion of one enantiomer into the other) are poorly understood. To better understand these processes, we investigated the chiral fungicide, triadimefon, which undergoes racemization in soils, water, and organic solvents. Nuclear magnetic resonance (NMR) and gas chromatography / mass spectrometry (GC/MS) techniques were used to measure the rates of enantiomerization and racemization, deuterium isotope effects, and activation energies for triadimefon in H2 O and D2 O. From these results we were able to determine that: 1) the alpha-carbonyl carbon of triadimefon is the reaction site; 2) cleavage of the C-H (C-D) bond is the rate-determining step; 3) the reaction is base-catalyzed; and 4) the reaction likely involves a symmetrical intermediate. The B3LYP/6-311 + G** level of theory was used to compute optimized geometries, harmonic vibrational frequencies, nature population analysis, and intrinsic reaction coordinates for triadimefon in water and three racemization pathways were hypothesized. This work provides an initial step in developing predictive, structure-based models that are needed to identify compounds of concern that may undergo racemization. Chirality 28:633-641, 2016. © 2016 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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