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The optimal conditions for immobilized enzyme invertase on chitosan were studied. Beside that, the aim of the present work was to find out if the processing with chitosan – invertase can affect some of the main honey quality paramenters – reductive sugar (RS), colour and antioxidant activity. RS content were analyzed by DNS method, colour parameters (L*, a*, b*) were established in the CIE system and antioxidant activity were analyzed by DPPH method. The results showed that the immobilized conditions were as follows: ratio chitosan/invertase 7/1 (w/w), invertase 0.8%, glutaraldehyde 4%, 50°C at 60 minutes, pH 4.5. After the treatments, there was significant (P |