Amperometric sarcosine biosensor based on hollow magnetic Pt–Fe3O4@C nanospheres
| Autor: | Haipeng Yang, Qingui Yang, Qiang Li, Hsing-Lin Wang, Shaoqing Chen, Li Na |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Detection limit
Sarcosine 010401 analytical chemistry Nanoparticle 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences Biochemistry Amperometry 0104 chemical sciences Analytical Chemistry Electrochemical gas sensor chemistry.chemical_compound chemistry Polyaniline Environmental Chemistry 0210 nano-technology Biosensor Spectroscopy Sarcosine oxidase Nuclear chemistry |
| Zdroj: | Analytica Chimica Acta. 1078:161-167 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2019.06.031 |
| Popis: | Sarcosine is a recently identified biomarker for prostate cancer. However, the rapid detection methods for sarcosine are relatively lack because of the low concentration and the presence of complicated interfering substances in serum or urine. In this manuscript, hollow nanospheres of Fe3O4 was synthesized and used as carrier to disperse Pt (Pt) nanoparticles. In order to achieve excellent electron transfer ability, we use polyaniline to coat Pt–Fe3O4 nanoparticles, and pyrolyze the polyaniline to carbon (C). Thus, hollow magnetic Pt–Fe3O4@C nanocomposites with good electron transfer ability are formed. The Pt–Fe3O4@C nanocomposites have high catalytic activity and stability. The nanocomposites were immobilized on glassy carbon electrode (GCE) to construct a nonenzyme hydrogen peroxide (H2O2) sensor (Pt–Fe3O4@C/GCE). We further construct a sensitive sarcosine biosensor by immobilizing sarcosine oxidase (SOx) on the Pt–Fe3O4@C/GCE. The high catalytic activity and good biocompatibility of Pt–Fe3O4@C nanocomposites greatly retained the bioactivity of immobilized SOx, and the prepared sarcosine biosensor has good electrocatalytic performance towards sarcosine. It has a linear detection range between 0.5 and 60 μM with a limit of detection (LOD) of 0.43 μM (the signal to noise ratio is 3), and the sensitivity is 3.45 nA μM−1 (48.8 nA μM−1 cm−2), which has the potential to be used for rapid screening of prostate cancer. |
| Databáze: | OpenAIRE |
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