Ceratonia siliqua L improved cryodamage of asthenozoospermic specimens: An experimental study
| Autor: | Fatemeh Javanmard, Ali Bidmeshkipour, Javad Ghasemian Yadegari |
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| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | International Journal of Reproductive BioMedicine (IJRM). |
| ISSN: | 2476-3772 2476-4108 |
| Popis: | Background: Sperm freezing is an important procedure in assisted reproductive technology. Freezing results in physical and chemical changes in the sperm. Ceratonia siliqua L (C.siliqua) is a tree that has antioxidant properties. Objective: This study aimed to investigate the effect of different concentrations of C.siliqua in a freezing medium on semen parameters, and some biochemical parameters in asthenozoospermic specimens. Materials and Methods: Forty asthenozoospermic specimens (semen specimens with motility < 32%) were obtained from men aged between 20-40 yr according to the World Health Organization criteria. Each sample was divided into 6 groups: I) fresh, II) control, III) 5, IV) 10, V) 20, and VI) 30 μg/ml C.siliqua extract were added to a freezing medium respectively. Then sperm parameters, malondialdehyde, total antioxidant capacity, reactive oxygen species, and sperm DNA assay were evaluated using related protocols after thawing. Results: Data analysis shows that sperm parameter, and total antioxidant capacity level increased at a concentration of 20 μg/ml of C. siliqua extract compared to the other concentrations of C.siliqua extract after cryopreservation and thawing (p < 0.001). Also, the sperm DNA fragmentation assay, reactive oxygen species, and malondialdehyde levels were significantly reduced by adding 20 μg/ml of C. siliqua extract to the sperm freezing medium compared to the other treated groups after cryopreservation (p < 0.001). Conclusion: C.siliqua extract significantly improved sperm parameters after cryopreservation and thawing in asthenozoospermic specimens, and the greatest impact was observed at the 20 μg/ml C.siliqua L extract concentration (p < 0.001). Key words: Asthenozoospermia, Ceratonia, Cryopreservation, Fertility preservation, Infertility, Male. |
| Databáze: | OpenAIRE |
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