Simple and sensitive fluorescence assay for acetylcholinesterase activity detection and inhibitor screening based on glutathione-capped gold nanoclusters
| Autor: | Zhi-Qi Zhang, Tian He, Meng Jin, Si-Si Liang, Rui-Ling Zhang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Stereochemistry
02 engineering and technology 010402 general chemistry 01 natural sciences Nanoclusters chemistry.chemical_compound Bromide Materials Chemistry medicine Electrical and Electronic Engineering Instrumentation Detection limit Chromatography Metals and Alloys Glutathione 021001 nanoscience & nanotechnology Condensed Matter Physics Fluorescence Acetylcholinesterase 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Thiocholine chemistry Tacrine 0210 nano-technology medicine.drug |
| Zdroj: | Sensors and Actuators B: Chemical. 253:196-202 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2017.06.136 |
| Popis: | A direct fluorescence turn-on method for simple and sensitive acetylcholinesterase (AChE) activity assay and AChE inhibitor screening has been developed by first using low-fluorescence glutathione-capped gold nanoclusters (GSH-AuNCs). The thiocholine produced by AChE-catalyzed hydrolysis of S-acetylthiocholine iodide could effectively enhance the fluorescence of GSH-AuNCs via Au S bond formation. In the presence of inhibitors, AChE activity was suppressed and thus fluorescence enhancement decreased. Therefore, AChE activity assay and inhibitor screening could be performed by measuring the fluorescence intensity of the system. The linear range of the AChE activity assay was 0–30 mU mL−1 with a limit of detection of 0.03 mU mL−1 (S/N = 3). The IC50 values of two inhibitors (tacrine and neostigmine bromide) were 42.92 nM and 37.04 nM, respectively. The developed protocol provides a simple and sensitive platform for assaying AChE activity and screening its inhibitors. |
| Databáze: | OpenAIRE |
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