Characterization of biodegradable cell micro and macro carriers based on recombinant spidroin

Autor:	M. M. Moisenovich, Debabov Vladimir G, D. G. Kozlov, Igor I. Agapov, L. I. Davydova, V. G. Bogush, A. Yu. Arkhipova, K. V. Sidoruk, Mikhail P. Kirpichnikov
Rok vydání:	2014
Předmět:	biology Scanning electron microscope Chemistry Spidroin Saccharomyces cerevisiae Adhesion Microstructure biology.organism_classification Applied Microbiology and Biotechnology Biochemistry In vitro law.invention Chemical engineering law Reagent Polymer chemistry Recombinant DNA
Zdroj:	Applied Biochemistry and Microbiology. 50:780-788
ISSN:	1608-3024 0003-6838
DOI:	10.1134/s000368381408002x
Popis:	Gels, microgels, and matrices were prepared based on a previously developed recombinant spidroin, 1F9, produced by Saccharomyces cerevisiae yeast strain; their physical, chemical, and biological properties were investigated. It was shown that microgels obtained from 2.5% hydrogel are sized in the range of 50–300 μm, with a predominance of particles of 50–150 μm in diameter. The microgel particles were stable in neutral, acidic, and alkaline media; the destruction of 50% (wt.) of the particles occurred in corrosive media (Fenton’s reagent) within three weeks. The microgel surface, which was studied by laser scanning confocal microscopy and scanning electron microscopy, has a complex relief in which there are both nano-(250–400 nm) and microstructures (3–7 μm). It was shown that particles of both microgel and matrices are capable of adhesion and support the proliferation of 3T3 murine fibroblasts in vitro.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9162d7f257b46357e0d57bd8ea2bcb45 https://doi.org/10.1134/s000368381408002x Zobrazit plný text záznamu Plný text ve formátu PDF