The characterization of growth factor activity in human brain

Autor:	Henry G. Friesen, T Tanaka, J M Rowe, Robert P. C. Shiu, J G Kellett
Rok vydání:	1981
Předmět:	biology medicine.diagnostic_test Isoelectric focusing Proteolysis Cell Biology Human brain Growth factor activity Fibroblast growth factor Biochemistry Myelin basic protein medicine.anatomical_structure Mitogen-activated protein kinase Pi biology.protein medicine Molecular Biology
Zdroj:	Journal of Biological Chemistry. 256:54-58
ISSN:	0021-9258 3736-3743
Popis:	The purification of fibroblast growth factor from bovine brain has been reported (Gospodarowicz, D., Bialecki, H., and Greenberg, G. (1978) J. Biol. Chem. 253, 3736-3743). Westall et al. (Westall, F. C., Lennon, V. A., and Gospodarowicz, D. (1978) Proc. Natl. Acad. Sci. U. S. A. 75, 4675-4678) showed that bovine brain fibroblast growth factor was composed of three fragments derived by limited proteolysis from myelin basic protein. In the present study using similar purification methods, we isolated a fraction enriched in growth factor activity from human brain. The mitogenic activity could not be resolved from myelin basic protein by chromatographic procedures but, upon isoelectric focusing, the mitogen and myelin basic protein were readily dissociated. At least two potent growth factors (pI values 7.2 to 7.4 and 8.1 to 8.6) were identified. Studies of a relatively crude basic extract of human brain suggested that the brain may contain a number of growth factors.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9160ee0a7b3e31813519b10c611a7d6b https://doi.org/10.1016/s0021-9258(19)70096-9 Zobrazit plný text záznamu