CIRCADIAN CHANGES IN RELATIVE ABUNDANCE OF TWO PHOTOSYNTHETIC TRANSCRIPTS IN THE MARINE MACROALGA KAPPAPHYCUS ALVAREZII (RHODOPHYTA)
| Autor: | Fabienne Goulard, Sabine Jacobsen, Klaus Lüning |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
0106 biological sciences
Cyanobacteria 0303 health sciences biology RuBisCO Circadian clock Plant Science Aquatic Science biology.organism_classification Photosynthesis 01 natural sciences Photosynthetic capacity 03 medical and health sciences Kappaphycus alvarezii Botany biology.protein 14. Life underwater Circadian rhythm Relative species abundance 030304 developmental biology 010606 plant biology & botany |
| Zdroj: | Journal of Phycology. 39:888-896 |
| ISSN: | 0022-3646 |
| Popis: | Circadian regulation of transcriptional gene expression has been shown for a variety of taxa throughout the eukaryotic kingdom and for prokaryotic cyanobacteria but not previously for macroalgae. We reported earlier a strong circadian rhythm of photosynthetic capacity in the red macroalga Kappaphycus alvarezii (Doty) Doty. To determine whether this rhythm is correlated with circadian regulation of steady-state transcript abundance, we analyzed transcripts of genes whose protein products are involved in photosynthesis. Transcript levels of two plastid-encoded operons, one for phycoerythrin α and β subunits and another for RUBISCO large and small subunits, were analyzed under light:dark cycles (LD) and under constant illumination (LL) for several days. Both transcripts exhibited diurnal regulation under LD conditions with maximum transcript abundance during early daytime to midday. Under LL conditions, circadian oscillations of steady-state mRNA abundance were detected for up to three cycles; thus, the analyzed transcripts exhibited diurnal and circadian regulation. Interestingly, peak abundance of the RUBISCO transcript shifted to subjective night under LL. To our knowledge, this is the first report demonstrating that circadian control of steady-state transcript abundance occurs in a macroalga. |
| Databáze: | OpenAIRE |
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