The effect of two fixation methods (TAF and DESS) on morphometric parameters of Aphelenchoides ritzemabosi

Autor:	Katarzyna Kowalewska, Aleksandra Bogumił, Aneta Chałańska, Tadeusz Malewski
Rok vydání:	2016
Předmět:	0301 basic medicine 03 medical and health sciences 030104 developmental biology Aphelenchoides biology Aphelenchoides ritzemabosi Animal Science and Zoology Anatomy Body size biology.organism_classification Fixation method Ecology Evolution Behavior and Systematics
Zdroj:	Zootaxa. 4083:297
ISSN:	1175-5334 1175-5326
DOI:	10.11646/zootaxa.4083.2.9
Popis:	Identification of nematode species by using conventional methods requires fixation of the isolated material and a suitable preparation for further analyses. Tentative identification using microscopic methods should also be performed prior to initiating molecular studies. In the literature, various methods are described for the preparation of nematodes from the genus Aphelenchoides for identification and microscopic studies. The most commonly used fixatives are formalin (Timm 1969; Szczygiel & Cid del Prado Vera 1981, Crozzoli et al. 2008, Khan et al. 2008), FAA (Wasilewska 1969; Vovlas et al. 2005, Khan et al. 2007) and TAF (Hooper 1958, Chizhov et al. 2006, Jagdale & Grewal 2006).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9149e0a063459931593755ba48c7db10 https://doi.org/10.11646/zootaxa.4083.2.9 Zobrazit plný text záznamu