Continuous Crystallization of Proteins in a Stirred Classified Product Removal Tank with a Tubular Reactor in Bypass
| Autor: | Christian Lohse, Nikolas von den Eichen, Dariusch Hekmat, Max Huber, Dirk Weuster-Botz |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Chromatography Materials science Precipitation (chemistry) Sedimentation (water treatment) technology industry and agriculture Nucleation Crystal growth General Chemistry equipment and supplies Condensed Matter Physics complex mixtures 01 natural sciences Suspension (chemistry) law.invention 03 medical and health sciences 030104 developmental biology Isoelectric point Chemical engineering law 010608 biotechnology General Materials Science Crystallization Protein crystallization |
| Zdroj: | Crystal Growth & Design. 17:4162-4169 |
| ISSN: | 1528-7505 1528-7483 |
| Popis: | A lab-scale stirred tank with a cooled tubular reactor in bypass was applied for continuous crystallization of lysozyme and a full-length therapeutic monoclonal antibody. The stirred tank was operated as a mixed suspension classified product removal crystallizer. Lysozyme was crystallized by a combination of cooling crystallization and salting-out. The antibody was crystallized by a combination of cooling crystallization and isoelectric crystallization. It was deduced that nucleation rates were enhanced when the protein solutions passed through the cooled tubular bypass. It was further deduced that crystal growth rates were enhanced in the stirred tank which was operated at a higher temperature compared to the tubular reactor. Classified product removal was possible by controlled sedimentation of protein crystals. The continuous crystallization system allowed a targeted control of crystal morphology and size. No sedimentation occurred in the tubular reactor and precipitation was avoided at all times. High... |
| Databáze: | OpenAIRE |
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