Inhibition of Na+, K+-ATPase activates swelling-induced taurine efflux in a human neuroblastoma cell line
| Autor: | Chiun Chien Huang, Ali Mobasheri, Samir Al-Adawi, R. J. Errington, Srisaila Basavappa, J. Clive Ellory |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Taurine
medicine.diagnostic_test Physiology medicine.drug_class Clinical Biochemistry Cell Cell Biology Biology Monoclonal antibody Molecular biology Ouabain law.invention chemistry.chemical_compound medicine.anatomical_structure chemistry Western blot Confocal microscopy law medicine Efflux Na+/K+-ATPase medicine.drug |
| Zdroj: | Journal of Cellular Physiology. 174:145-153 |
| ISSN: | 1097-4652 0021-9541 |
| Popis: | The Na+ pump (Na+, K+-ATPase) has been implicated in the regulation of many cellular functions, including cell volume regulation. The effects of inhibiting Na+ pump activity on cell volume and taurine efflux were evaluated in the human neuroblastoma cell line CHP-100. Cell volume changes monitored with the Coulter Multisizer technique and confocal microscopy showed that neuroblastoma cells exposed to ouabain swelled by 22 +/- 4% (n = 5). The rapid cell swelling was followed by regulatory volume decrease (RVD). In cells treated with ouabain, 14C-taurine efflux increased by 183 +/- 11% compared with controls. However, cells exposed simultaneously to ouabain and hypoosmotic solution resulted in a 14C-taurine efflux of 207 +/- 18%. Western blot and immunofluorescence microscopy with specific monoclonal antibodies for the catalytic alpha isoforms of Na+, K+-ATPase demonstrated high levels of the ubiquitously expressed alpha1 and the neuronal-specific alpha3. Ouabain-binding data showed that CHP-100 cells express approximately 3 x 10(5) pump units/cell. The present data indicate that efflux of taurine may be involved during volume recovery subsequent to blockade of Na+, K+-ATPase in CHP-100 cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text