Steroidal glycosides from the roots of Cynanchum stauntonii and their effects on the expression of iNOS and COX-2
| Autor: | Yi Dai, Jian-Xin Liu, Liang Liu, Hua Zhou, Chang-Zhi Lai, Jin-Shan Tang, Zhen-Qiang Mu, Xin-Sheng Yao, Jun Wu, Shu-Wen Pang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
chemistry.chemical_classification
biology Lipopolysaccharide 010405 organic chemistry Cynanchum stauntonii Stereochemistry Chemistry Glycoside Plant Science Carbon-13 NMR Inhibitory postsynaptic potential 01 natural sciences Biochemistry 0104 chemical sciences Nitric oxide synthase 010404 medicinal & biomolecular chemistry chemistry.chemical_compound biology.protein Macrophage Agronomy and Crop Science Heteronuclear single quantum coherence spectroscopy Biotechnology |
| Zdroj: | Phytochemistry Letters. 16:38-46 |
| ISSN: | 1874-3900 |
| Popis: | Six new steroidal glycosides, named stauntosides O-T ( 1 – 6 ), along with eight known compounds ( 7 – 14 ), were obtained from the 95% aqueous ethanol extract of the roots of Cynanchum stauntonii . Their chemical structures were elucidated by IR, HR ESI-MS/MS, 1 H- and 13 C NMR, 1 H- 1 H COSY, HSQC, HSQC-TOCSY, and HMBC spectroscopic analyses, which showed interesting 13,14:14,15-disecopregnane-type or 14,15-secopregnane-type C 21 steroidal glycosides. The glycosides’ anti-inflammatory effects were investigated by detecting the inhibitory effects of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) on RAW246.7 murine macrophage cells stimulated by lipopolysaccharide (LPS). Our results showed that compounds 1 , 5 , 8 , 9 , 11 , and 13 could significantly inhibit iNOS expression, and compounds 5 and 7 could clearly reduce COX-2 expression in RAW246.7 cells stimulated by LPS compared to cells stimulated with LPS and not treated with other compounds. Thus, compounds 1 , 5, 7–9, 11 , and 13 have the potential to mediate anti-inflammatory effects, with compound 5 having a greater anti-inflammatory effect than the other compounds. |
| Databáze: | OpenAIRE |
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