CD4-CD8- T cells from mice with collagen arthritis display aberrant expression of type l K+ channels
| Autor: | S Grissmer, D C Hanson, E J Natoli, M D Cahalan, K G Chandy |
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| Rok vydání: | 1990 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 145:2105-2109 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Expression of voltage-gated K+ channels in mAb-defined T cell subsets from normal mice and mice with experimental autoimmune arthritis was studied with the patch-clamp whole-cell recording technique in combination with fluorescence microscopy. CD4+CD8- Th cells from DBA/1 LacJ mice with type II collagen arthritis expressed low levels of type n K+ channels, and CD4-CD8+ T cells (cytotoxic) showed small numbers of type l or n' K+ channels, like their phenotypic counterparts in normal mice. CD4-CD8-Thy-1.2+ (double negative or DN) T cells from the diseased mice, however, displayed an abundance of type l K+ channels compared to DN T cells in normal mice, or mice immunized with CFA. Furthermore, the aberrant expression of type l K+ channels correlated with the presence of active disease. DN T cells from mice with SLE, type-1 diabetes mellitus, and experimental allergic encephalomyelitis, also exhibited a high number of type l K+ channels. These results suggest that expression of numerous type l K+ channels may be a useful marker for DN T cells associated with these autoimmune disorders. |
| Databáze: | OpenAIRE |
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