Prevalence of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae Causing Bloodstream Infections in Cancer Patients from Southwest of Iran
| Autor: | Mehrandokht Sirous, Bijan Keikhaei, Sakineh Seyed-Mohammadi, Effat Abbasi Montazeri, Azar Dokht Khosravi, Morteza Saki |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.drug_class Klebsiella pneumoniae medicine.medical_treatment 030106 microbiology Antibiotics Microbiology 03 medical and health sciences 0302 clinical medicine Medicine Pharmacology (medical) 030212 general & internal medicine Pharmacology biology business.industry Cancer Enterobacter biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification medicine.disease Enterobacteriaceae Citrobacter freundii Infectious Diseases Serratia marcescens Beta-lactamase business |
| Zdroj: | Infection and Drug Resistance. 13:1319-1326 |
| ISSN: | 1178-6973 |
| DOI: | 10.2147/idr.s254357 |
| Popis: | Introduction This study aimed to evaluate the frequency rate of extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-PE) causing bloodstream infections (BSIs) in cancer patients referred to one of the major referral hospitals in Ahvaz city, southwest Iran. Materials and methods In this study, 1700 blood cultures were collected from 610 cancer patients suspected to have BSI from October 2016 to August 2017 referred to the Shafa cancer hospital, Ahvaz, southwest of Iran. The blood culture bottles were incubated aerobically at 35-37oC for 24 hours and then sub-cultured on routine microbiology culture media. The bacterial colonies were identified using standard tests. The antibiotic susceptibility testing was achieved by the disc-diffusion method. The phenotypic detection of ESBLs was carried out by the combination disc-diffusion test (CDDT). Finally, the polymerase chain reaction (PCR) was performed to investigate the presence of bla TEM, bla CTX, bla SHV, and bla PER genes. Results The prevalence of BSI in cancer patients was 16.4% (100/610). Gram-negative rods with rate of 74% (74/100) were the most prevalent bacteria. The frequency of Enterobacteriaceae family was 21% including Escherichia coli (n: 8), Klebsiella pneumoniae (n: 6), Enterobacter spp. (n: 5), Citrobacter freundii (n: 1), and Serratia marcescens (n: 1). All isolates were multidrug-resistant (resistance to three or more antibiotics). The results of CDDT showed that 42.8% (9/21) of Enterobacteriaceae isolates had a positive ESBL test of which 100% (9/9) indicated positive band for at least one of the ESBL genes by PCR method. The bla CTX-M and bla TEM genes were detected in 38% (8/21) and 23.8% (5/21) of isolates, respectively, while the bla SHV and bla PER were not detected in any isolates. Conclusion Based on the results, surveillance, and antibiotic stewardship programs should be implemented for cancer patients to prevent the spread of more ESBL-PE that have limited therapeutically choices. |
| Databáze: | OpenAIRE |
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