Applications of Click Chemistry Reaction for Proteomics Analysis
| Autor: | Jin-Ming Zhang, Si-Yu Gao, Yuan-Dong Xie, Yi Li, Ning Liu, Fang-Ling Zhan |
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| Rok vydání: | 2020 |
| Předmět: |
Reaction mechanism
Chemistry 010401 analytical chemistry High selectivity Nanotechnology 02 engineering and technology 021001 nanoscience & nanotechnology Proteomics 01 natural sciences Small molecule 0104 chemical sciences Analytical Chemistry Structure and function Molecular level otorhinolaryngologic diseases Click chemistry 0210 nano-technology |
| Zdroj: | Chinese Journal of Analytical Chemistry. 48:431-438 |
| ISSN: | 1872-2040 |
| DOI: | 10.1016/s1872-2040(20)60007-x |
| Popis: | Cu(I)-catalyzed azide-alkyne specific reaction, popularly known as the “click chemistry reaction”, is defined as an important molecular connection method, which can realize simple and efficient connection between specific small molecule groups at the molecular level. Because of its advantages such as high efficiency, high selectivity, mild conditions and few side reactions, click chemistry reaction has been widely used in proteomics analysis, including identification of newly synthesized proteins and the identification of various post-translational modified proteins. Therefore, in-depth study of the reaction mechanism of click chemistry reaction in proteomics is very important for understanding the structure and function of proteins. This review highlights the progress of several important applications of click chemistry in proteomics analysis in recent years, and discusses the future trends of the click chemistry in proteomics. |
| Databáze: | OpenAIRE |
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