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This study shows the contribution of filamentous actin (F-actin) to sensing applied mechanical vibration in mouse fibroblastoid cells experimentally. A living body may adapt to mechanical stimulus from surroundings, but its mechanism has not been revealed. It is assumed that this adaptation process may be caused by the reaction of each cell consisting the body. Several experiments have shown that cells cultured under some mechanical vibration increase about 30% in cell number compared with those without any vibration. After these findings, the linker of the nucleus and cytoskeleton and one of the transmembrane protein, integrin α5β1, has been reported to be the essential factor of sensing vibration. Then, we hypothesize that cells sense mechanical vibration through the nucleus, F-actin, integrin, and fibronectin connectivity. In this paper, we focus on the contribution of F-actin which binds nucleus and integrin for sensing mechanical vibration. The mouse fibroblastoid cells were cultured under mechanical vibration. To assess the contribution of F-actin to sensing vibration, one of the fungal toxins named cytochalasin D (CD) which was actin polymerization inhibitors was included in the medium of cells. Four groups, that is, with CD under static or vibration and without CD under static or vibration, were prepared. By comparing the cell number between these groups after confluent concentration state, the increase of cell number by vibration was observed just in case without CD. This result indicated that the organized F-actin was an essential factor of sensing the applied vibration. |
