Establishment of Human- and Mouse-Derived Gastric Primary Epithelial Cell Monolayers from Organoids

Autor:	Nina Bertaux-Skeirik, Emma L. Teal, Jayati Chakrabarti, Loryn Holokai, Yana Zavros
Rok vydání:	2018
Předmět:	0301 basic medicine Physiological function Chemistry Regeneration (biology) Epithelium In vitro Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure 030220 oncology & carcinogenesis Monolayer medicine Organoid Stem cell
Zdroj:	Methods in Molecular Biology ISBN: 9781493985999
DOI:	10.1007/978-1-4939-8600-2_15
Popis:	Organoid cultures generated from gastrointestinal tissues have been an invaluable advancement for in vitro studies of physiological function and disease. Here we present a comprehensive protocol for the establishment and culture of human- and mouse-derived 3-dimensional gastric organoids transferred to 2-dimensional gastric epithelial cell monolayers. We introduce two methods that include the establishment of monolayers from: (1) intact organoids, and (2) single cells dissociated from intact organoids.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::90f5394fc04f3e7004c82d22163764d7 https://doi.org/10.1007/978-1-4939-8600-2_15 Zobrazit plný text záznamu