Identification by MALDI-TOF Mass Spectrometry of Mercury-resistant Bacteria Associated with the Rhizosphere of an Apple Orchard
| Autor: | Juan Caballero Pérez, George H. Jones, Raquel Louvan Rivera Zarazúa, Jaime Pérez Cruz, Julio Alfonso Cruz Medina, Diana Laura Juarez Arteaga, Iván Arvizu Hernández, Zyanya Gloria Mena Casas, Camille Calzada Urquiza, Juan Campos-Guillén, Miguel Angel Ramos López, Adriana Anahíd Contreras García |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Rhizosphere Volatilisation 030106 microbiology Nucleic acid sequence chemistry.chemical_element Mass spectrometry Rhizobacteria Microbiology Mercury (element) 03 medical and health sciences Horticulture 030104 developmental biology Bioremediation chemistry Botany Earth and Planetary Sciences (miscellaneous) Environmental Chemistry Orchard General Environmental Science |
| Zdroj: | Geomicrobiology Journal. 34:176-182 |
| ISSN: | 1521-0529 0149-0451 |
| DOI: | 10.1080/01490451.2016.1165316 |
| Popis: | In this work, mercury-resistant bacterial strains were isolated from the rhizosphere of an apple orchard, growing in a soil with high levels of mercury (Nuevo San Joaquin, Queretaro State, Mexico). Analysis of the soil in this region by the Cold Vapor Atomic Absortion Spectroscopy method showed that it contained 637 ± 51 mg mercury per kg. Mercury accumulation by fresh apples from this orchard amounted to 15.44 ± 4.33 mg/kg. The bacterial isolates were identified by application of proteomic technique of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). They were found to be strains of Bacillus muralis and Bacillus simplex. All strains showed the ability to catalyze the volatilization of Hg as measured via the nonradioactive X-ray method. In all strains merR and merA genes were detected by polymerase chain reaction. Nucleotide sequence analysis showed that merR from B. simplex was 435 bp in length and that its sequence was similar to merR sequences reporte... |
| Databáze: | OpenAIRE |
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