Determination of ambroxol in dog plasma by high-performance liquid chromatography and UV detection

Autor:	I. Ürmös, Imre Klebovich, K. Balogh Nemes, József Szúnyog, B. Dalmadi Kiss
Rok vydání:	2000
Předmět:	Chromatography Chemistry Calibration curve Organic Chemistry Clinical Biochemistry Ambroxol Analytical chemistry Plasma Biochemistry High-performance liquid chromatography Biological fluid Analytical Chemistry medicine Sample preparation Solid phase extraction Uv detection medicine.drug
Zdroj:	Chromatographia. 51:S217-S220
ISSN:	1612-1112 0009-5893
DOI:	10.1007/bf02492809
Popis:	A new sensitive HPLC-UV method has been developed and validated for the determination of amboroxol in dog plasma enabling the investigation of a newly developed 75 mg ambroxol-containing retard capsule of EGIS Pharmaceuticals Ltd., Budapest, Hungary. A gradient method was used for removing the longer retained plasma components of no interest. The separation was performed on a BDS Hypersil C18 (5 μm, 250×2.1 mm) analytical column, supplied with a 10 mm guard column containing the same packing material. The detection was performed at 210 nm. The calibration curve was linear in the range 25–2000 ng·mL−1. Nerisopam (EGIS-6775) was used as internal standard.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::90e0d643a3f97fc13f4ec72e026dd928 https://doi.org/10.1007/bf02492809 Zobrazit plný text záznamu Full text from SpringerLink