Morphologic structure and immunohistochemical analysis of vaginal wall in women with pelvic organ prolapse
| Autor: | Andrey Kaprin, I.V. Vasina, V B Filimonov, R V Vasin, M V Mnikhovich, A A Kostin |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Hysterectomy biology medicine.diagnostic_test business.industry medicine.medical_treatment Urology Connective tissue Urinary incontinence Vimentin General Medicine medicine.disease medicine.anatomical_structure Fibrosis Biopsy medicine biology.protein Immunohistochemistry medicine.symptom business Type I collagen |
| Zdroj: | Urologiia. :12-20 |
| ISSN: | 1728-2985 |
| Popis: | AIM to study a correlation between clinical stage of pelvic organ prolapse (POP), a histological structure and results of immunohistochemical study of the vaginal wall were evaluated. MATERIALS AND METHODS A total of 60 peri- and postmenopausal women (average age 61.9+/-8.4 years) with POP of stage II-IV, according to the POP-Q classification, or with stress urinary incontinence and cystocele of stage I-II, who undergone to surgical treatment, were included in the study. During a procedure, a biopsy from the anterior vaginal wall was taken. Depending on the stage of POP, patients was divided into two groups. In the group 1, 30 patients with stage I and II of POP were included, while group 2 included 30 women with POP of stage III and more. The control group (group 3) consisted of 20 patients without POP (mean age 63.4+/-11.0 years) who underwent a hysterectomy due to to other indications. A histological and immunohistochemical studies of vaginal wall tissue was performed in order to determine the tissue content of collagen type I and III; matrix metalloproteinases 1 and 2 (MMP-1 and MMP-2), a tissue inhibitor of metalloproteinases 1 (TIMP-1), vimentin and smooth muscle actin. RESULTS In contrast to two other groups, in group 2 there were significant changes in the connective tissue. Collagen has a form of fibrous mass with areas of reduced content. In addition, scarring areas with an increase in the content of type III collagen, a decrease in the amount of type I collagen and elastic fibers with significant fragmentation, were seen. Moreover, in patients with severe POP (III-IV), degradation of collagen fibers with a decrease in connective tissue strength and elasticity was detected. Women with POP had a low ratio of type I:III collagen. Analysis of the collagen content in the vaginal wall in patients with mild POP (I-II) revealed a significant increase in the level of collagen type I (p=0.0003) and a decrease in the content of type III (p=0.045), compared to patients with more severe POP (III-IV). The level of MMP-1 and MMP-2 in women with POP was higher, than in control group by 1.7 times (p |
| Databáze: | OpenAIRE |
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