Popis: |
This study aims the determination of antifungal activity and the flavonoids content of extracts from carpophores of some wild and cultivated fungi. The work was conducted on Wild fungi (Fomes fomentarius, Hericiumerinaceus, Schizophyllum commune, Plerotus ostreatus were collected in Tunisia from Ain Drahem (Northern west), Tbainia (Northern west), Kef Rand (Northern east), and Tunis regions. Cultivated mushroom were Pleurotus ostreatus and Lentinus edodes (Shiitake) species from Ain Drahem. Cultivation of these last two species was realized on logs of Populus sp. and Quercus canariensis respectively. The total flavonoid content of crude extract was determined by the aluminium chloride colorimetric method. The antifungal activity was tested against five fungal strains: Alternaria alternate, Penicillium olsonii, Ulocladium atrum, Phytophtora nicotianae, Aspergillus fumigates. Results showed significant differences between the fungal species and between the two extracts studied. Methanol extracts showed the highest flavonoid amount. The most important value was reached by methanol extract of Wild Plerotus ostreatus (2.3 mg RE/ml). Aqueous extract of studied mushrooms showed the most important antifungal activities. Fomes fomentarius Ain Drahem and cultivated P. ostreatus aqueous extracts showed the highest inhibitory rate again Alternaria alternata species (70% and 63.33% respectively). |