On the ultrastructural localization of cell surface sialyl residues versus anionic sites
| Autor: | E.A. Bayer, B. Morein, E. Skutelsky, S. Hoglund |
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| Rok vydání: | 1978 |
| Předmět: | |
| Zdroj: | Proceedings, annual meeting, Electron Microscopy Society of America. 36:294-295 |
| ISSN: | 2690-1315 0424-8201 |
| Popis: | Membrane-associated sialic acids(SA) are considered to have an important role in a variety of cell sur ace interactions. Visualization of SA sites and their ultrastructural quantitative evaluation have heverally been based on the coulombic interaction between anionic sites of sialyl carboxyl groups with polycationic,electron dense markers, e.g. colloidal ferric hydroxide or cationized ferritin(CF). We have recently demonstrated an alternative method, whereby periodate-induced biotinylation (PIB) of unfixed cells can be used for specific localization of SA in thin sections by ferritin-conjugated avidin (FAv). It was further shown that PIB does not affect the surface anionic charge, since the latter is still available to CF-staining. In order to determine the role of anionic sites on the distribution and configuration of cell surface sialoglycoproteins and or sialoglycolipids, we have compared the topographic distribution of attached FAv or CF particles on normal and pathological blood cells,following successive treatment with sodium periodate and biotin hydrazide. |
| Databáze: | OpenAIRE |
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