Golgi Distribution of Lyn to Caveolin- and Giantin-Positive cis-Golgi Membranes and the Caveolin-Negative, TGN46-Positive trans-Golgi Network
| Autor: | Noritaka Yamaguchi, Naoto Yamaguchi, Takao Morinaga, Aya Okamoto |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pharmacology Pharmaceutical Science General Medicine Brefeldin A Golgi apparatus environment and public health Transport protein Cell biology 03 medical and health sciences chemistry.chemical_compound symbols.namesake 030104 developmental biology 0302 clinical medicine Secretory protein chemistry Membrane protein LYN 030220 oncology & carcinogenesis Caveolin symbols Tyrosine kinase |
| Zdroj: | Biological & Pharmaceutical Bulletin. 41:142-146 |
| ISSN: | 1347-5215 0918-6158 |
| DOI: | 10.1248/bpb.b17-00681 |
| Popis: | Src-family tyrosine kinases, classified as cytosolic enzymes, have crucial roles in regulating cell proliferation, differentiation, migration and cell-shape changes. Newly synthesized Lyn, a member of Src-family kinases, is biosynthetically accumulated at the cytoplasmic face of caveolin-containing Golgi membranes via posttranslational lipid modifications and then transported to the plasma membrane. However, the precise intra-Golgi localization of Lyn remains elusive. By means of a 19°C block-release technique and short-term brefeldin A treatment, we show here that the distribution of Lyn is not monotonously spread within the Golgi but selectively intensified in two distinct membrane compartments: giantin- and caveolin-positive membranes and trans-Golgi network protein (TGN)46-positive but caveolin-negative membranes. Furthermore, Lyn exits the Golgi from the caveolin-positive cis-Golgi cisternae or the caveolin-negative trans-Golgi network. These results suggest that Lyn moves apart from caveolin, a secretory protein, within the Golgi during Lyn's trafficking to the plasma membrane. |
| Databáze: | OpenAIRE |
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