Autor: | van Gastelen, Sanne, Muizelaar, Wouter, Bani, Paolo, Kuhla, Björn, Larsen, Mogens, Yáñez-Ruiz, David |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: | |
DOI: | 10.5680/mcpb008 |
Popis: | There are different purposes for collecting rumen fluid, e.g. assessment of rumen fermentation characteristics and rumen microbiota, as well as ex vivo incubations for feed analysis. Collecting rumen content through the fistula from rumen cannulated ruminants is considered the reference method for the collection of representative samples of rumen digesta, but access to surgically-modified animals is not universal and restricted to research facilities. Hence, a less invasive alternative has been developed: the oral stomach tubing (OST) technique. The OST technique allows rumen fluid to be obtained through the oesophagus. Several types of devices from different manufacturers can be used for sampling rumen fluid via the OST technique, all with specific advantages and disadvantages. The decision for the type of device should always follow the purpose of the rumen fluid collection and fit best with the type of ruminant, the familiarity of the animal with handling, and the amount of sample required. Chapter of the Living Handbook Methods in cattle physiology and behaviour – Recommendations from the SmartCow consortium |
Databáze: | OpenAIRE |
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