Sensitive Immunoassay for Fumonisin B1 in Corn
| Autor: | Bing D. M. Dang, Jupiter M. Yeung, Lorraine A. Robinson, D. B. Prelusky, Marc E. Savard |
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| Rok vydání: | 1996 |
| Předmět: |
Detection limit
Fumonisin B1 Chromatography biology medicine.diagnostic_test Chemistry Toxin food and beverages General Chemistry medicine.disease_cause Human serum albumin chemistry.chemical_compound Polyclonal antibodies Immunoassay Fumonisin biology.protein medicine General Agricultural and Biological Sciences Mycotoxin medicine.drug |
| Zdroj: | Journal of Agricultural and Food Chemistry. 44:3582-3586 |
| ISSN: | 1520-5118 0021-8561 |
| Popis: | Polyclonal antibodies against fumonisin B 1 (FB 1 ) were produced in rabbits after immunization with FB 1 conjugates. Protection of the amino group prior to coupling the carboxylic acid of FB 1 to human serum albumin, and then deprotecting the amine before immunization, generated high-affinity antibodies. The antibodies produced were specific to fumonisins and their hydrolyzed products (HFB 1 , HFB 2 ) but not to any other mycotoxins commonly found in grains. The relative cross-reactivities of FB 1 , fumonisin B 2 (FB 2 ), fumonisin B 3 (FB 3 ), HFB 1 , and HFB 2 were 100%, 55%, 13%, 11%, and 3%, respectively. Using these antibodies, a sensitive indirect competitive ELISA was developed. The detection limit and IC 50 for FB 1 in water were 0.05 and 0.66 ng/mL, respectively. The linear range was determined to be 0.05-0.75 ng/mL. The method was applied directly to 75% methanol extracts of corn. Recoveries of FB 1 were better than 87% from 10 to 10 000 ng/g at five levels of fortification. The detection limit in corn was 5 ng/g. |
| Databáze: | OpenAIRE |
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