Analysis of metal-containing proteins by gel electrophoresis and synchrotron radiation X-ray fluorescence
| Autor: | M. Kühbacher, G. Weseloh, Arndt Knöchel, Mehmet Ozaslan, H. Bertelsmann, A. Kyriakopoulos |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Detection limit
Gel electrophoresis Chromatography Chemistry Health Toxicology and Mutagenesis Public Health Environmental and Occupational Health Synchrotron radiation X-ray fluorescence Pollution Fluorescence Analytical Chemistry Metal Nuclear Energy and Engineering visual_art visual_art.visual_art_medium Radiology Nuclear Medicine and imaging Polyacrylamide gel electrophoresis Spectroscopy Combined method |
| Zdroj: | Journal of Radioanalytical and Nuclear Chemistry. 259:473-477 |
| ISSN: | 0236-5731 |
| Popis: | For the analysis of metal-containing proteins, sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) has been combined with synchrotron radiation X-ray fluorescence (SRXRF). In a pilot study the applicability of this combined method was tested in the analysis of metalloaded apoazurin and of the selenoproteins in rat testis homogenate. It was shown that it can be applied in the determination of the major stable binding forms of trace elements. After further improvement of the limits of detection the method will allow the analysis of trace element-containing proteins present in the samples at low concentrations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|