MOLECULAR IDENTIFICATION OF Tinospora sinensis BY ITS2 SEQUENCE ANALYSIS

Autor:	Paul Nilesh, D. M. Desai, Devendra Lingojwar, M. R. Raut, A. K. Gangawane, R. S. Khanapurkar
Rok vydání:	2012
Předmět:	Genetics genomic DNA Intergenic region Phylogenetic tree Sequence analysis Phylogenetics parasitic diseases Amplicon Biology Genome DNA sequencing
Zdroj:	International Journal of Molecular Biology. 3:55-57
ISSN:	0976-0490 0976-0482
DOI:	10.9735/0976-0482.3.2.55-57
Popis:	Intergenic spacer region 2 (ITS2) between 5.8S RNA and 28S RNA is well established genomic region for molecular identification of plant as potential universal barcode. The Present studies deals with molecular identification of Tinospora sinensis, by a unique ITS2 re- gion. Briefly, the plant genomic DNA isolated by CTAB method and targeted for amplification of ITS2 region by eukaryotic conserved primers i.e. RM5.8s1F and RM 28s R for generation of 400 bp amplicon followed by DNA sequencing. Sequence analysis by BLAST search on NCBI database and phylogenetic studies suggests 96% identity with published sequence of Tinospora sinensis, i.e. intraspecies identity and 92% identity with other species of Tinospora genera i.e. T. malabarica. Phylogenetic analysis by MEGA4.1 suggest more close relationship with Tinospora sinensis Thus, this is a first report of ITS2 based molecular detection of Tinospora sinensis from Indian soil.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::90a25a44945186dfddc5b122f36a1859 https://doi.org/10.9735/0976-0482.3.2.55-57 Zobrazit plný text záznamu