Comparative study of BMP-2 gene delivery to Human adipose tissue-derived mesenchymal stem cells with Turbofect and Polyethylenimine

Autor:	M. A. Syachina, I. A. Nedorubova, T. B. Bukharova, A. A. Kulakov, A. V. Vasilyev, D. V. Goldshtein
Rok vydání:	2021
Předmět:	Polyethylenimine chemistry.chemical_compound animal structures Chemistry embryonic structures fungi Mesenchymal stem cell Adipose tissue Gene delivery Bone morphogenetic protein 2 Cell biology
Zdroj:	IOP Conference Series: Earth and Environmental Science. 677:042024
ISSN:	1755-1315 1755-1307
Popis:	Genetic modification of mesenchymal stem cells (MSCs) with plasmid encoding the bone morphogenetic protein 2 gene (BMP2) is a crucial task in the development of gene therapy and tissue engineering technologies for bone regeneration. The low transfection efficiency of MSC cultures is a limitation for practical applications and makes it necessary to search for optimal protocols that ensure efficient gene delivery while maintaining sufficient cell viability. Comparison of two transfecting reagents (TurboFect and Polyethylenimine) shows that TurboFect is the most effective for MSCs transfection. A higher level of target gene BMP2 expression and osteogenic differentiation can be achieved using the TaqRFP-N-BMP2 plasmid compared with pcDNA3-BMP2.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::909dd56cc3ee9a5d2d299bc2cde3e342 https://doi.org/10.1088/1755-1315/677/4/042024 Zobrazit plný text záznamu