| Popis: |
Collagen and elastin are two fibrous proteins which occur in varying amounts in practically every tissue. They are particularly abundant in the connective tissues. Collagen, and possibly elastin, is extremely widespread throughout the phylogenetic system. In the last 15 years there has been a widespread interest in these proteins, including physiological and clinico-pathological studies which depend almost exclusively on an accurate determination of the amount of these proteins in various tissues. In a good proportion of these studies, misunderstandings of the limitations of the methods used have reduced the value of the published data. This article is therefore concerned with a critical evaluation of the various methods of quantitating collagen and elastin, pointing out the pitfalls to be encountered, and recommending the methods suitable for the particular situations that are currently under investigation in many laboratories. |
